By T.K. Ghose ,A. Fiechter, N.Blakebrough
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Additional info for Advances in Biochemical Engineering, Volume 009: Microbial Processes
Thus the relationship between the specific growth rate and the specific concentration of hydrocarbon per unit cell mass, S • X - l , was investigated. The results are shown in Fig. 24, which indicates that the saturation kinetics is valid for the specific growth rate and the specific concentration of hydrocarbon per unit cell mass. The Lineweaver-Bulk plots of specific Mechanism of Liquid Hydrocarbon Uptake by Microorganisms and Growth Kinetics 30 51 °i/ 20 a. 1¢ 'IP ::i, I0 Fig. 23. 15 & "2 Fig.
Oxygen uptake rate, QO2, on accommodation-form n-tetradecane for C. 063 g]l Mechanism of Liquid Hydrocarbon Uptake by Microorganismsand Growth Kinetics 39 for C. intermedia Qo~ (AH)max = 6 x 10 -3 (mole 02)- (g cell) - l • h - l and for C. 3 x 10 -2 (mole 02) • (g cell) -1 • h - l • The rates of oxygen uptake at various concentrations of alanine-precultured cells were measured with a definite initial concentration of accommodation-form n-tetradecane, 5000 ppm. These rates of oxygen uptake are shown in Fig.
Mechanism of Liquid Hydrocarbon Uptake by Microorganisms and Growth Kinetics 33 Table 1. Assumptions made for the calculation of the thickness of the n-hexadeeane layer attached to the cell surface 181 Mean cell diam. Cell vol. /mg dry wt. 10 -~5 m 3 5 • 107 2" I0-10 m 3 4 • I0 -t8 m 3 Osumi et al.  investigated the ultrastructure of the cell surface of Candida tropicalis and that o f C. albicans growing on n-alkanes using field emission scanning electron microscopy. A curious protrusion o f 1 0 0 - 2 0 0 mm in diameter was observed on the surface of the yeast cells.
Advances in Biochemical Engineering, Volume 009: Microbial Processes by T.K. Ghose ,A. Fiechter, N.Blakebrough